Services

ProBiostructures offers comprises a complete range of X-ray crystallography and cryo-electron microscopy research, called “gene to structure”, that is enriched with biophysical and biochemical characterization of target-ligand interactions.

Our services primarily support structure-based drug design, hit to lead and structure-based lead optimization. A remarkable advantage is unique expertise in the crystallization of protein-nucleic acid complexes and novel approach to structural biology enhanced by cryo-EM analysis.

Preparation of expression constructs

usługa 1

  1. Design of the expression constructs and expression strategy (determination of the optimal construct boundaries, the selection of the appropriate expression vector, purification tag etc.).
  2. Preparation of constructs for expression.

   

Recombinant protein production in bacteria, yeast, LEXSY, baculovirus-insect cells, and mammalian cells 

usuga 2

  1. Recombinant protein expression tests.
  2. Optimization of protein expression and purification.
  3. Large scale protein overexpression and chromatographic purification.

   

X-ray crystallography of protein or protein–ligand complexes

usługa 3

  1. Crystallization and crystal optimization.
  2. Crystallization of protein and protein – inhibitor complex.
  3. X-ray diffraction measurements and data collection.
  4. Structure determination and refinement.

   

Single-particle cryo-electron microscopy (cryo-EM) with the goal of determining high-resolution structures of macromolecules with a molecular weight ≥ 150 kDa

  1. Negative stain EM – preliminary negative stain analysis.
  2. Negative stain EM – optimization of the sample homogeneity.
  3. Cryo-EM screening.
  4. Final cryo-EM analysis (high resolution data collection).
  5. Structure determination and refinement.

   

Biophysical and biochemical assays

  1. Biophysical/biochemical characterization of target-ligand interactions (e.g., Biacore SPR, ITC, fluorescence anisotropy).
  2. Assay design and optimization.

   

The service contains detailed project discussion and the offer of optimal solutions for the client. We also offer signing of an NDA (a non-disclosure agreement).

DATA PROTECTION
For secure data exchange with our partners, we are using Office 365 Cloud Services–OneDrive for Business and SharePoint Online. If the company has a different system, then we are ready to open an independent sub-account for it or use another system.

STARTING NEW PROJECTS AND RISK SHARING
Each project starts with a feasibility assessment. Afterward, preliminary experiments are conducted at a price of the operational costs (risk sharing). When ProBiostructures delivers satisfactory project results, the final price consists entirely of a success fee.

 

Please contact us to receive a detailed offer (contact).