Services

Leaderna offers a complete range of X-ray crystallography and cryo-EM techniques, called Gene-to-Structure, complemented with biophysical and biochemical characterization of target-ligand interaction.

Our services primarily support structure-based drug design, hit to lead and structure-based lead optimization.
We offer an extensive expertise in structural analysis of protein:nucleic acid complexes with unique cross-linking technology for the most challenging projects.

 

Preparation of expression constructs

usługa 1

  1. Design of the expression constructs and expression strategy (determination of the optimal construct boundaries, the selection of the appropriate expression vector, purification tag etc.).
  2. Preparation of constructs for expression.

   

Recombinant protein production 

usuga 2

  1. Recombinant protein expression tests.
  2. Optimization of protein expression and purification.
  3. Large scale protein overexpression and chromatographic purification.

   

X-ray crystallography (Gene-to-Structure)

usługa 3

  1. Crystallization and crystal optimization.
  2. Crystallization of protein and protein – inhibitor complex.
  3. X-ray diffraction measurements and data collection.
  4. Structure determination and refinement.

   

Single-particle cryo-EM

  1. Negative stain EM – preliminary negative stain analysis.
  2. Negative stain EM – optimization of the sample homogeneity.
  3. Cryo-EM screening.
  4. Final cryo-EM analysis (high resolution data collection).
  5. Structure determination and refinement.

   

Biophysical and biochemical assays

  1. Biophysical/biochemical characterization of target-ligand interactions (e.g., Biacore SPR, ITC, fluorescence anisotropy).
  2. Assay design and optimization.

   

DATA PROTECTION

We treat data protection and information security just as seriously as our research work. We have policies and procedures that safeguard privacy of data and promote good security habits among our employees. Our information systems can be used to exchange data and information with our customers in fast, reliable and secure way.

STARTING NEW PROJECTS AND RISK SHARING

The service contains detailed project discussion and the offer of optimal solutions for the client.

Due to the experimental work frame of drug discovery and our services, each project starts with feasibility assessment. Afterward, preliminary experiments are conducted at a price of the operational costs (risk sharing). When Leaderna delivers satisfactory project results, the final price consists entirely of a success fee.

CONTROL AND CALCULATION DURING THE PROJECT

We want to be as flexible as possible for our clients. We designed a special task calculator that allows us to keep the spirit and the manner of scientific research while carrying out projects for the industry. Thanks to this solution, clients can have full control over the project and cooperate with Leaderna as if in their own internal laboratory.

 

Please contact us to receive a detailed offer (contact) or place an order via Science Exchange (button below).

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